CST, 48241T, HADHB (F8N5B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HADHB. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, HADHB (F8N5B) Rabbit Monoclonal Antibody (CST #48241) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HADHB (F8N5B) Rabbit Monoclonal Antibody recognizes endogenous levels of total HADHB protein. This antibody does not cross-react with HADHA protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val110 of human HADHB protein. Background Mitochondrial trifunctional enzyme, also known as mitochondrial trifunctional protein (MTP), catalyzes three reactions in the fatty acid β-oxidation pathway. It has two different types of subunits: α/HADHA and β/HADHB. HADHA possesses the 2,3-enoyl-CoA hydratase (ECH) and 3-hydroxyacyl-CoA dehydrogenase (HACD) activities, while HADHB has 3-ketoacyl-CoA thiolase activity (1-3). Studies show that HADHA stimulates ketone body production through fatty acid β-oxidation. β-hydroxybutyrate, one of the ketone bodies, inhibits gluconeogenesis (1). Furthermore, studies have identified mutations in that cause MTP deficiency, an autosomal recessive disorder severely restricting long-chain fatty acid oxidation (4-6). Alternate Names 2-enoyl-Coenzyme A (CoA) hydratase, beta subunit; 3-ketoacyl-CoA thiolase; 3-ketoacyl-Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein, beta subunit; Acetyl-CoA acyltransferase; Beta-ketothiolase; ECHB; HADHB; hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta; hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit; hydroxyacyl-Coenzyme A (CoA) dehydrogenase, beta subunit; hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit; MGC87480; mitochondrial trifunctional enzyme, beta subunit; mitochondrial trifunctional protein, beta subunit; MSTP029; MTPB; TP-beta; Trifunctional enzyme subunit beta, mitochondrial Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Rabbit IgG