CST, 48277SF, BRM (D9E8B) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying BRM. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, BRM (D9E8B) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #48277) is ready to ship. Product Usage Information This product is the carrier free version of product #11966. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 11966 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity BRM (D9E8B) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total BRM protein. This antibody does not cross-react with BRG1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly264 of human BRM protein. Background ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9). BRM and BRG1 are also considered to be tumor suppressors and their expression levels are severely reduced in several cancer cell lines (10-13). Alternate Names ATP-dependent helicase SMARCA2; BAF190; BAF190B; brahma homolog; BRG1-associated factor 190B; BRM; FLJ36757; global transcription activator homologous sequence; hBRM; hSNF2a; MGC74511; NCBRS; Probable global transcription activator SNF2L2; Protein brahma homolog; SMARCA2; SMCA2; SNF2; SNF2-alpha; SNF2-like 2; SNF2/SWI2-like protein 2; SNF2A; SNF2L2; SNF2LA; Sth1p; sucrose nonfermenting 2-like protein 2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2; SWI2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG