CST, 48298T, Anillin (F6M2V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying anillin. Validated for WB,WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Anillin (F6M2V) Rabbit Monoclonal Antibody (CST #48298) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:100 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Anillin (F6M2V) Rabbit Monoclonal Antibody recognizes endogenous levels of total anillin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala14 of human anillin protein. Background Anillin/ANLN is an actin-binding protein with roles in cytokinesis and cell migration (1). Anillin is highly expressed in human cancer and is a potential prognostic biomarker and therapeutic target (2). In hepatocellular carcinoma (HCC), anillin expression is correlated with poor prognosis, and anillin deficiency induces multinucleated cells, apoptosis, and DNA damage checkpoints (3). Anillin knockdown in HCC leads to cell growth inhibition through impairment of cytokinesis (4). Anillin expression is negatively regulated by DNA damage in a p53-dependent manner (5). Alternate Names Actin-binding protein anillin; Anillin; anillin actin binding protein; anillin, actin binding protein; ANLN; DKFZp779A055; FSGS8; scra; Scraps Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 150-180 Source/Isotype: Rabbit IgG