CST, 48309SF, Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PI3K p85 alpha (Tyr467) phosphate/PI3K p55 (Tyr199) phosphate/PI3K p85 beta (Tyr464) phosphate. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #48309) is ready to ship. Product Usage Information This product is the carrier free version of product #17366. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 17366 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of p85/p55 protein only when phosphorylated at Tyr467/Tyr199 (Tyr458/Tyr199 in mouse). Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr467 (equivalent to Tyr458 in mouse) of human p85α protein. Background Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP ). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). Protein extracts from 3T3-Src cells were profiled by PhosphoScan to identify phosphotyrosine peptides. Tyr458 of PI3K p85 and Tyr199 of PI3K p55 were among 180 phosphopeptides and 185 phosphotyrosine sites identified (5). Alternate Names 100% homology to SWISS-PROT Q92569; AGM7; DKFZp686P05226; FLJ41892; GRB1; IMD36; MPPH; MPPH1; p55; p55-GAMMA; P55G; p55PIK; p85; p85-ALPHA; p85-BETA; P85A; P85B; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphatidylinositol 3-kinase, regulatory, 1; phosphoinositide-3-kinase regulatory subunit 1; phosphoinositide-3-kinase regulatory subunit 2; phosphoinositide-3-kinase regulatory subunit 3; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit beta; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphoinositide-3-kinase, regulatory subunit 2 (beta); phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit 3 (gamma); phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma); phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit beta; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3-kinase subunit p85-beta; PI3K regulatory subunit alpha; PI3K regulatory subunit beta; PI3K regulatory subunit gamma; PIK3R1; PIK3R2; PIK3R3; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit beta; PtdIns-3-kinase regulatory subunit gamma; PtdIns-3-kinase regulatory subunit p55-gamma; PtdIns-3-kinase regulatory subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-beta Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 60, 85 Source/Isotype: Rabbit IgG