CST, 4830S, Calmodulin Antibody

Polyclonal Antibody for studying Calmodulin. Validated for Western Blotting. Highly specific and rigorously validated in-house, Calmodulin Antibody (CST #4830) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Calmodulin Antibody detects endogenous levels of total calmodulin protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Glu140 of human calmodulin. Antibodies are purified by peptide affinity chromatography. Background Calmodulin is a ubiquitously expressed small protein mediating many cellular effects such as short-term and long-term memory, nerve growth, inflammation, apoptosis, muscle contraction and intracellular movement (1). Upon binding of four Ca ions, calmodulin undergoes conformational changes, allowing this complex to bind to and activate many enzymes including protein kinases, protein phosphatases, ion channels, Ca pumps, nitric oxide synthase, inositol triphosphate kinase, and cyclic nucleotide phosphodiesterase (2,3). Since calmodulin binds Ca in a cooperative fashion, small changes in cytosolic Ca levels lead to large changes in the level of active calmodulin and its target proteins (4). Alternate Names CALM; CALM1; CALM2; CALM3; CALML2; Calmodulin; calmodulin 1; calmodulin 1 (phosphorylase kinase, delta); calmodulin 2 (phosphorylase kinase, delta); calmodulin 3 (phosphorylase kinase, delta); Calmodulin-1; Calmodulin-2; Calmodulin-3; CAM; CAM1; CAM2; CAM3; CAMB; CAMC; CAMI; CAMIII; CPVT4; DD132; LQT14; PHKD; phosphorylase kinase subunit delta; phosphorylase kinase, delta subunit; prepro-calmodulin 1 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 17 SOURCE: Rabbit