CST, 4835S, PHD-2/Egln1 (D31E11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PHD-2 (Egln1). Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PHD-2/Egln1 (D31E11) Rabbit Monoclonal Antibody (CST #4835) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PHD-2/Egln1 (D31E11) Rabbit Monoclonal Antibody detects endogenous levels of total PHD-2/Egln1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val226 of human PHD-2/Egln1 protein. Background PHD-1 (Egln2), PHD-2 (Egln1), and PHD-3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL/ubiquitin pathway (1,2). PHD-1 is highly expressed in a wide array of tissues, whereas PHD-2 and PHD-3 are expressed mainly in heart and skeletal muscle (1,3). The mRNA levels of PHD are upregulated by HIF through the hypoxia-response element under low oxygen conditions (4-7). These three enzymes also exhibit different peptide specificity target proteins, PHD-1 and PHD-2 can hydroxylate both proline 402 and proline 564, but PHD-3 can only hydroxylate proline 564 (2,8). In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin (3,9). Alternate Names C1orf12; DKFZp761F179; ECYT3; Egl nine homolog 1; egl nine homolog 1 (C. elegans); egl nine-like protein 1; egl-9 family hypoxia inducible factor 1; EGLN1; HALAH; HIF prolyl hydroxylase 2; HIF-PH2; HIF-prolyl hydroxylase 2; HIFPH2; HPH-2; HPH2; Hypoxia-inducible factor prolyl hydroxylase 2; PHD2; Prolyl hydroxylase domain-containing protein 2; SM-20; SM20; zinc finger MYND domain-containing protein 6; ZMYND6 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG