CST, 48362T, MIA40/CHCHD4 (F8J4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CHCHD4/MIA40. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MIA40/CHCHD4 (F8J4L) Rabbit Monoclonal Antibody (CST #48362) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MIA40/CHCHD4 (F8J4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total MIA40/CHCHD4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala128 of human MIA40/CHCHD4 protein. Background Mitochondrial intermembrane space import and assembly protein 40 (MIA40), also known as coiled-coil-helix-coiled-coil-helix domain-containing protein 4 (CHCHD4), is a chaperone and oxidoreductase in the mitochondrial intermembrane space (IMS). It is an import receptor for various intermembrane space proteins and has a key role in their oxidative folding (1,2). Studies show that the IMS protein AIFM1 mediates the import of MIA40/CHCHD4. Furthermore, AIFM1 forms a stable complex with MIA40/CHCHD4. This complex is crucial for the import of MIA40/CHCHD4 substrates into the IMS (1). In addition, the mitochondrial inner-membrane-associated protein C9orf72 is imported into the IMS via AIFM1-MIA40/CHCHD4 pathway. C9orf72 then stabilizes the assembly of mitochondrial complex I (3). Alternate Names CHCHD4; coiled-coil-helix-coiled-coil-helix domain containing 4; Coiled-coil-helix-coiled-coil-helix domain-containing protein 4; FLJ31709; MIA40; Mitochondrial intermembrane space import and assembly protein 40; TIMM40; translocase of inner mitochondrial membrane 40 homolog Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG