CST, 48367T, DKK1 (D5V6L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DKK1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DKK1 (D5V6L) Rabbit Monoclonal Antibody (CST #48367) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 88194 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity DKK1 (D5V6L) Rabbit Monoclonal Antibody recognizes endogenous levels of total DKK1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DKK1 protein. Background Dickkopf (DKK) family proteins consist of four members (DKK1, DKK2, DKK3, and DKK4) that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6). DKK1 was initially identified as an inducer of head formation in Xenopus embryos (7) and plays an important role in the regulation of bone mass (8-10). Research studies indicate that increased levels of DKK1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers (11,12), while DKK1 expression is decreased in malignant melanoma and colorectal cancers (13,14). Alternate Names dickkopf 1 homolog; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; dickkopf WNT signaling pathway inhibitor 1; Dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; Dickkopf-related protein 1; Dkk-1; DKK1; hDkk-1; SK Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28-40 Source/Isotype: Rabbit IgG