CST, 4836S, Phospho-MARK Family (Activation Loop) Antibody

Polyclonal Antibody for studying MARK2 (Thr208) phosphate/MARK3 (Thr211) phosphate/MARK1 (Thr215) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-MARK Family (Activation Loop) Antibody (CST #4836) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-MARK Family (Activation Loop) Antibody detects endogenous levels of phosphorylated MARK family members, MARK1 at threonine 215, MARK2 at threonine 208, and MARK3 at threonine 234 (A.K.A. 211 in isoforms 3-6) . This antibody does not react with MARK4. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding threonine 215 of human MARK1. Antibodies were purified by protein A and peptide affinity chromatography. Background Microtubule associated proteins regulate the stability of microtubules and control processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking (1). The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases was identified based on their ability to phosphorylate microtubule-associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARK proteins phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimer's disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4). The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7). Alternate Names C-TAK1; Cdc25C-associated protein kinase 1; CTAK1; ELKL motif kinase 1; ELKL motif kinase 2; EMK-1; EMK-2; EMK1; EMK2; KIAA1477; KP78; MAP/microtubule affinity-regulating kinase 1; MAP/microtubule affinity-regulating kinase 2; MAP/microtubule affinity-regulating kinase 3; MARK; MARK1; MARK2; MARK3; MGC126512; MGC126513; MGC99619; microtubule affinity regulating kinase 1; microtubule affinity regulating kinase 2; microtubule affinity regulating kinase 3; PAR-1; Par-1a; Par-1b; Par-1c; PAR1 homolog; PAR1 homolog b; PAR1 homolog c; PAR1A; Par1b; Par1c; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK; Serine/threonine-protein kinase MARK1; Serine/threonine-protein kinase MARK2; Serine/threonine-protein kinase p78; testicular tissue protein Li 117; VIPB Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80 to 95 SOURCE: Rabbit