CST, 4839T, RecQL1 (Q1N3) Mouse Monoclonal Antibody

Monoclonal Antibody for studying RecQL1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, RecQL1 (Q1N3) Mouse Monoclonal Antibody (CST #4839) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:80 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity RecQL1 (Q1N3) Mouse Monoclonal Antibody detects endogenous levels of total RecQL1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human RecQL1 recombinant protein. Background The RecQ family is a group of DNA helicases that play an important role in global genomic stability (1). Mutations in three of the five known human RecQ proteins (BLM, WRN and RECQL4) give rise to clinically distinct disorders that are characterized by features such as premature aging and predisposition to cancer (2,3). The clinical distinction of each disease associated with these mutations points to distinct roles that members of this helicase family play in DNA metabolism. RecQL1 is the most abundant protein of the RecQ family and was the first family member to be discovered. No disease associations have been reported with RecQL1 and its biological activities are not well understood (4). It has recently been shown that depletion of RecQL1 negatively affects genomic maintenance and cellular proliferation - which may point to a role in DNA damage repair and cell cycle progression (5,6). Upregulation of RecQL1 along with other RecQ family members has been reported in cells in response to oncogenic viral infection (7). Alternate Names ATP-dependent DNA helicase Q1; DNA helicase Q1-like; DNA helicase, RecQ-like type 1; DNA-dependent ATPase Q1; RecQ helicase-like; RecQ like helicase; RecQ protein-like; RecQ protein-like (DNA helicase Q1-like); RecQ protein-like 1; RECQ1; RECQL; RECQL1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Mouse IgG1