CST, 48481T, GDF15/MIC1 (E4D3K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Mic-1/GDF15. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, GDF15/MIC1 (E4D3K) Rabbit Monoclonal Antibody (CST #48481) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity GDF15/MIC1 (E4D3K) Rabbit Monoclonal Antibody recognizes endogenous levels of total GDF15/MIC1 protein. This antibody reacts with monomeric and dimeric forms of pro-GDF15/MIC1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human GDF15/MIC1 protein. Background Macrophage inhibitory cytokine-1 (Mic-1), also termed GDF15 (1), PTGF-β (2), PLAB (3), PDF (4), and NAG-1 (5), is a divergent member of the transforming growth factor-β (TGF-β) superfamily (6). Like other family members, Mic-1 is synthesized as an inactive precursor that undergoes proteolytic processing involving removal of an N-terminal hydrophobic signal sequence followed by cleavage at a conserved RXXR site, generating an active C-terminal domain that is secreted as a dimeric protein. Mic-1 is highly expressed in the placenta and is also dramatically increased by cellular stress, acute injury, inflammation, and cancer. In the brain, Mic-1 is found in the choroid plexus and is secreted into the cerebrospinal fluid (7). It is also a transcriptional target of the p53 tumor suppressor protein and may serve as a biomarker for p53 activity (8,9). During tumor progression, Mic-1 has various effects on apoptosis, differentiation, angiogenesis, and metastasis, and may also contribute to weight loss during cancer (10,11). Alternate Names GDF-15; GDF15; growth differentiation factor 15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC1; NAG-1; non-steroidal anti-inflammatory drug-activated gene-1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; Placental bone morphogenetic protein; Placental TGF-beta; Prostate differentiation factor; PTGF-beta; PTGFB Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG