CST, 4848T, N-WASP (30D10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying N-WASP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, N-WASP (30D10) Rabbit Monoclonal Antibody (CST #4848) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity N-WASP (30D10) Rabbit Monoclonal Antibody recognizes endogenous levels of total N-WASP protein. This antibody does not cross-react wth the hematopietic protein, WASP. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding the sequence of human N-WASP. Background Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8). Alternate Names Actin nucleation-promoting factor WASL; DKFZp779G0847; MGC48327; N-WASP; Neural Wiskott-Aldrich syndrome protein; NWASP; WASL; WASP like actin nucleation promoting factor; WASPB; Wiskott-Aldrich syndrome gene-like; Wiskott-Aldrich syndrome gene-like protein; Wiskott-Aldrich syndrome like; Wiskott-Aldrich syndrome-like Specification REACTIVITY: H M R Hm Mk B SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit