CST, 48490S, THEMIS2 (F2V8Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying THEMIS2. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, THEMIS2 (F2V8Q) Rabbit Monoclonal Antibody (CST #48490) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity THEMIS2 (F2V8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total THEMIS2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr605 of human THEMIS2 protein. Background Thymocyte-expressed molecule involved in selection protein 2 (THEMIS2) is a member of the THEMIS family of proteins, initially identified as a scaffold protein involved in TLR signaling and tumor cell differentiation (1). THEMIS2 contains two conserved cysteine-based domains, a proline-rich region, and a nuclear localization signal (1-4). LPS stimulation triggers THEMIS2 phosphorylation and activation, facilitating its interaction with several key MAP kinase proteins and action in several immune cell types (1). THEMIS2 is a key player in T cell development following TCR engagement (2-4). In natural killer (NK) cells, THEMIS2 regulates memory function and formation, and can regulate antitumor activity by inhibiting receptors that activate NK cell signaling (2,3). THEMIS2 is involved in the positive selection of B cells and germinal center B cells by self and foreign antigens. THEMIS2 has been shown to lower the threshold for B cell activation by low-avidity, but not high-avidity, antigens (4). Recent research has focused on the role of THEMIS2 in cancer biology (5-8). Its transcription is estrogen-responsive, and THEMIS2 protein expression is implicated in breast and ovarian cancers (6-8). Elevated THEMIS2 protein levels are seen in ovarian and endometrial carcinomas (7,8). Significantly increased THEMIS2 expression was shown in breast cancer cells (8), underscoring its potential as a target for cancer therapies. Alternate Names basement membrane-induced; C1orf38; chromosome 1 open reading frame 38; ICB-1; ICB1; Induced by contact to basement membrane 1 protein; Protein ICB-1; Protein THEMIS2; THEMIS2; THMS2; thymocyte selection associated family member 2; Thymocyte-expressed molecule involved in selection protein 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG