CST, 4852S, PCTAIRE 1 Antibody

Polyclonal Antibody for studying Pctaire1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, PCTAIRE 1 Antibody (CST #4852) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PCTAIRE 1 Antibody detects endogenous levels of total PCTAIRE 1. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg98 of human PCTAIRE 1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Three distinct PCTAIRE isoforms (PCTAIRE 1, PCTAIRE 2 and PCTAIRE 3) have been identified in humans and belong to the CDK family of serine/threonine protein kinases. These proteins have a core kinase domain flanked by unique amino- and carboxy-terminal domains. CDK proteins are known to regulate the cell cycle. All three PCTAIRE isoforms are abundantly expressed and catalytically active in post-mitotic brain, suggesting that they may function in processes other than cell division (1). PCTAIRE 1 is a cytoplasmic phosphoprotein whose kinase activity peaks in G2 and S phase (2). While one study indicates that noncovalent interactions with a regulatory component (such as a cyclin) are necessary for catalytic activity of PCTAIRE 1, others show that the monomeric protein is fully active (3). The Cdk5/p25 complex phosphorylates PCTAIRE 1 at Ser95, enhancing its kinase activity (4). Alternate Names CDK16; Cell division protein kinase 16; cyclin dependent kinase 16; Cyclin-dependent kinase 16; FLJ16665; PCTAIRE; PCTAIRE-motif protein kinase 1; PCTAIRE1; PCTGAIRE; PCTK1; Serine/threonine-protein kinase PCTAIRE-1; testis secretory sperm-binding protein Li 224n Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 65 SOURCE: Rabbit