CST, 4859S, Phospho-Mre11 (Ser676) Antibody

Polyclonal Antibody for studying Mre11 (Ser676) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Mre11 (Ser676) Antibody (CST #4859) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Mre11 (Ser676) Antibody detects endogenous levels of Mre11 only when phosphorylated at Ser676. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser676 of human Mre11. Antibodies are purified by protein A and peptide affinity chromatography. Background Mre11, originally described in genetic screens from the yeast in which mutants were defective in meiotic recombination (1), is a central part of a multisubunit nuclease composed of Mre11, Rad50 and Nbs1 (MRN) (2,3). The MRN complex plays a critical role in sensing, processing and repairing DNA double strand breaks. Defects lead to genomic instability, telomere shortening, aberrant meiosis and hypersensitivity to DNA damage (4). Hypomorphic mutations of Mre11 are found in ataxia-telangiectasia-like disease (ATLD), with phenotypes similar to mutations in ATM that cause ataxia-telangiectasia (A-T), including a predisposition to malignancy in humans (5). Cellular consequences of ATLD include chromosomal instability and defects in the intra-S phase and G2/M checkpoints in response to DNA damage. The MRN complex may directly activate the ATM checkpoint kinase at DNA breaks (6). Phospho-Mre11 (Ser676) Antibody is directed to a site that was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser676 was discovered using an ATM/ATR substrate antibody and was shown to be induced by UV treatment (7). Please visit PhosphoSitePlus , CST's modification site knowledgebase, at www.phosphosite.org for more information. Alternate Names AT-like disease; ATLD; DNA recombination and repair protein; Double-strand break repair protein MRE11; Double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; Meiotic recombination 11 homolog 1; Meiotic recombination 11 homolog A; MRE11; MRE11 double strand break repair nuclease A; MRE11 homolog 1; MRE11 homolog A; MRE11 homolog A, double strand break repair nuclease; MRE11 homolog, double strand break repair nuclease; MRE11 homolog, double strand break repair nuclease A; MRE11 meiotic recombination 11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); MRE11 meiotic recombination 11-like protein A; MRE11A; MRE11B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 81 SOURCE: Rabbit