CST, 4860S, WASP Antibody

Polyclonal Antibody for studying WASP. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, WASP Antibody (CST #4860) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity WASP Antibody detects endogenous levels of total WASP protein. This antibody does not cross-react with N-WASP. Species Reactivity: Human, Mouse Source / Purification Polyclonal Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro175 of human WASP protein. Antibodies are purified using protein A and peptide affinity chromatography. Background Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8). Alternate Names Actin nucleation-promoting factor WAS; eczema-thrombocytopenia; IMD2; SCNX; THC; THC1; thrombocytopenia 1 (X-linked); WAS; WASp; WASP actin nucleation promoting factor; WASPA; Wiskott-Aldrich syndrome (eczema-thrombocytopenia); Wiskott-Aldrich syndrome protein Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 60 human, 62 mouse SOURCE: Rabbit