CST, 48625T, DUSP1/MKP1 (E8L7D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DUSP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DUSP1/MKP1 (E8L7D) Rabbit Monoclonal Antibody (CST #48625) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DUSP1/MKP1 (E8L7D) Rabbit Monoclonal Antibody recognizes endogenous levels of total DUSP1/MKP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val347 of human DUSP1/MKP1 protein. Background MAP kinases are inactivated by dual-specificity protein phosphatases (DUSPs) that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli, and cellular localization. DUSPs, also known as MAPK phosphatases (MKPs), specifically dephosphorylate both threonine and tyrosine residues in MAPK P-loops and have been shown to play important roles in regulating the function of the MAPK family (1,2). At least 13 members of the family (DUSP1-10, DUSP14, DUSP16, and DUSP22) display unique substrate specificities for various MAP kinases (3). MAPK phosphatases typically contain an amino-terminal rhodanese-fold responsible for DUSP docking to MAPK family members and a carboxy-terminal catalytic domain (4). These phosphatases can play important roles in development, immune system function, stress responses, and metabolic homeostasis (5). In addition, research studies have implicated DUSPs in the development of cancer and the response of cancer cells to chemotherapy (6). Alternate Names CL 100; CL100; dual specificity phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1; DUSP1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein-tyrosine phosphatase CL100; PTPN10; serine/threonine specific protein phosphatase; VH1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG