CST, 48633T, KLC1 (D2T2R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KLC1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, KLC1 (D2T2R) Rabbit Monoclonal Antibody (CST #48633) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity KLC1 (D2T2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total KLC1 protein. This antibody also cross-reacts with an unidentified protein of 25 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly443 of human KLC1 protein. Background Kinesins are heterotetrameric motor proteins that transport cargo along microtubule tracks toward their plus ends (anterograde direction) in an ATP-dependent manner. Two heavy chains contain the motor activity, while two kinesin light chains act as adaptor proteins that may be required for binding of specific cargo and/or regulation of heavy chain catalytic activity. The amino terminus of kinesin light chain 1 (KLC1) binds to kinesin heavy chains while the KLC1 carboxy-terminal tetratricopeptide repeat (TPR) domain binds cargo (1-3). Phosphorylation of KLC1 at Ser521 by AMPK may regulate insulin granule dynamics (4,5). Research studies identify a KLC1-ALK fusion protein in human lung adenocarcinoma, and this finding may provide insight for future therapeutics (6). Alternate Names kinesin 2 60/70kDa; Kinesin light chain 1; KLC; KLC 1; KLC1; KNS2; KNS2A; medulloblastoma antigen MU-MB-2.50; MGC15245 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 65, 75 Source/Isotype: Rabbit IgG