CST, 4863S, Phospho-Zyxin (Ser142/143) Antibody

Polyclonal Antibody for studying zyxin (Ser142/Ser143) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Zyxin (Ser142/143) Antibody (CST #4863) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Zyxin (Ser142/143) Antibody recognizes endogenous levels of zyxin protein only when phosphorylated at Ser142, Ser143 or both residues. Species Reactivity: Human, Mouse, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser142 of human zyxin protein. Antibodies are purified using protein A and peptide affinity chromatography. Background The zyxin family of proteins includes LIMD1, ajuba, trip6 and zyxin, each of which contains three LIM domains at the carboxy-terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1). Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2), and in regulation of adhesion and migration, possibly through recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4). Alternate Names ESP-2; HED-2; ZYX; Zyxin; Zyxin-2 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 78 SOURCE: Rabbit