CST, 4866S, VDAC Antibody

Polyclonal Antibody for studying VDAC1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, VDAC Antibody (CST #4866) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:75 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity VDAC Antibody detects endogenous levels of total VDAC protein. Species Reactivity: Human, Mouse, Rat, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human VDAC-1. Antibodies are purified using protein A and peptide affinity chromatography. Background Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (1). In addition, this channel plays a role in apoptotic signaling. The change in mitochondrial permeability characteristic of apoptosis is mediated by Bcl-2 family proteins, which bind to VDAC, altering the channel kinetics (2). Homodimerization of VDAC may be a mechanism for changing mitochondrial permeability and supporting release of cytochrome c (3). In mammalian cells, there are three VDAC isoforms, VDAC1, which is the most widely expressed isoform, as well as VDAC2 and VDAC3 (4,5). Alternate Names hVDAC1; MGC111064; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; PORIN; Porin 31HL; Porin 31HM; PORIN-31-HL; sperm binding protein 1a; VDAC; VDAC-1; VDAC1; voltage dependent anion channel 1; voltage-dependent anion channel 1; Voltage-dependent anion-selective channel protein 1 Specification REACTIVITY: H M R B SENSITIVITY: Endogenous MW (kDa): 32 SOURCE: Rabbit