CST, 4868S, HSP40 Antibody

Polyclonal Antibody for studying HSP40. Validated for WB,IP,IF,F. Highly specific and rigorously validated in-house, HSP40 Antibody (CST #4868) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity HSP40 Antibody detects endogenous levels of total HSP40 protein. This antibody does not cross-react with other HSPs. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to Glu300 of human HSP40/Hdj1. Antibodies are purified by protein A and peptide affinity chromatography. Background HSP40 and HSP40-like proteins represent a large family of chaperone proteins that are homologous to DnaJ protein (1). These proteins are classified into three subtypes based on their structures. The common feature of the family is a conserved J domain, which is usually located at the amino terminus of proteins and responsible for their association with HSP70 (1,2). Human HSP40, also known as Hdj1, belongs to subtype II that contain a unique Gly/Phe-rich region (2). HSP40 family proteins bind unfolded proteins, prevent their aggregation, and then deliver them to HSP70 (2,3). Another major function of HSP40 is to stimulate ATPase activity of HSP70, which causes conformational change of the unfolded proteins (4,5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 or components of the protein degradation machinery such as CHIP and BAG-1, which either leads to protein folding or degradation, respectively (6). Alternate Names DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; DnaJ heat shock protein family (Hsp40) member B1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB1; DNJB1; hDj-1; HDJ1; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; Heat shock protein 40; HSP40; HSPF1; Human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit