CST, 4869S, HSP60 (D85) Antibody

Polyclonal Antibody for studying HSP60. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, HSP60 (D85) Antibody (CST #4869) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity HSP60 (D85) Antibody detects endogenous levels of total HSP60 protein. This antibody does not cross-react with other HSPs. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Asp85 of human HSP60. Antibodies are purified by protein A and peptide affinity chromatography. Background In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8). Alternate Names 60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; CH60; Chaperonin 60; CPN60; epididymis secretory sperm binding protein; GROEL; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; heat shock protein family D (Hsp60) member 1; HLD4; HSP-60; HSP60; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13 Specification REACTIVITY: H M R Mk Dm SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit