CST, 48701T, PTPRF/LAR (E9B9S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAR. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PTPRF/LAR (E9B9S) Rabbit Monoclonal Antibody (CST #48701) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PTPRF/LAR (E9B9S) Rabbit Monoclonal Antibody recognizes endogenous levels of total PTPRF/LAR protein. This antibody detects the E-subunit of the processed PTPRF/LAR protein. Weak signal is detected in mouse and rat. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PTPRF/LAR protein. Background Receptor type protein tyrosine phosphatase F (PTPRF, LAR) is a transmembrane PTP that helps to regulate insulin signaling, cell proliferation and cell migration. The PTPRF protein is composed of an extracellular segment that contains several Ig-like and fibronectin (Fn-III) domains, a transmembrane region and a pair of cytoplasmic phosphatase domains (1,2). Functional studies reveal that the membrane-associated D1 phosphatase domain is responsible for substrate dephosphorylation, while the D2 domain is important for substrate specificity (3). PTPRF negatively regulates insulin signaling through dephosphorylation of insulin receptor and insulin receptor substrate (4). This phosphatase activates the pro-apoptotic DAPK serine/threonine kinase by removing a phosphate at Tyr491/492, while the kinase Src replaces the phosphate to inactivate DAPK at the same time it down regulates PTPRF expression (5). PTPRF is commonly found at focal adhesions where it interacts with liprin, which localizes the phosphatase to the membrane, and the Rac/Rho family GTPase Trio (6). Localization of PTPRF at adherens junctions results in PTPRF modification of β-catenin, which inhibits cell migration by limiting the amount of available cytosolic β-catenin (7). Alternate Names BNAH2; FLJ43335; FLJ45062; FLJ45567; LAR; LCA-homolog; leukocyte antigen-related (LAR) PTP receptor; leukocyte antigen-related tyrosine phosphatase; Leukocyte common antigen related; protein tyrosine phosphatase receptor type F; protein tyrosine phosphatase, receptor type, F; protein tyrosine phosphatase, receptor type, F polypeptide; PTPRF; receptor-linked protein-tyrosine phosphatase LAR; Receptor-type tyrosine-protein phosphatase F Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150, 213 Source/Isotype: Rabbit IgG