CST, 48750T, CD20 (E7B7T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD20. Validated for WB,IP,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CD20 (E7B7T) Rabbit Monoclonal Antibody (CST #48750) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 92688 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD20 (E7B7T) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD20 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with human CD20 recombinant protein. Background B-lymphocyte antigen CD20 (also known as MS4A1; Membrane-spanning 4-domains subfamily A member 1) is a cell surface phosphoprotein involved in the regulation of B cell activation and proliferation (1,2). It is commonly used as a marker to identify B cells and is expressed throughout B cell development, up until their differentiation into plasma cells. CD20 has no known ligand, and its expression and function are largely conserved between human and mouse (1-3). Evidence suggests that CD20 is necessary for store operated calcium (SOC) entry, which leads to elevated cytoplasmic calcium levels required for B cell activation (4,5). Anti-CD20 antibody immunotherapy depletes B cells by activation of the innate monocytic network and is a common treatment for B cell lymphomas, leukemias, and autoimmune diseases (6). Alternate Names B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; B1; Bp35; CD20; CD20 antigen; CD20 receptor; CVID5; LEU-16; Leukocyte surface antigen Leu-16; membrane spanning 4-domains A1; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969; MS4A1; MS4A2; S7 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 30-40 Source/Isotype: Rabbit IgG