CST, 48789S, UPP1 Antibody

Polyclonal Antibody for studying UPP1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, UPP1 Antibody (CST #48789) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UPP1 Antibody recognizes endogenous levels of total UPP1 protein. This antibody may also detect a non-specific band at approximately 45 kDa in a limited number of cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys174 of human UPP1 protein. Antibodies are purified by peptide affinity chromatography. Background Uridine phosphorylases (UPP1, UPP2) are metabolic enzymes that catalyze the reversible phosphorolytic cleavage of uridine into uracil and ribose-1-phosphate, which can subsequently serve as cellular energy sources or as substrates for nucleotide biosynthesis via pyrimidine salvage pathways (1). Altered pyrimidine metabolism has been reported in multiple cancer subtypes (2-5) and is hypothesized to be associated with cellular metabolic reprogramming events that enable enhanced tumor growth and metastasis (6,7). Notably, UPP1 was identified as one of the enzymes involved in the metabolic activation of the anti-neoplastic agent 5-fluorouracil (5-FU) (8,9). Somewhat paradoxically, numerous UPP1 inhibitors have been developed to reduce the adverse side effects of 5-FU treatment by elevating physiological levels of uridine during 5-FU administration (10). Alternate Names UDRPASE; UP; UPASE; UPase 1; UPP; UPP1; UrdPase 1; Uridine phosphorylase 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 33 SOURCE: Rabbit