CST, 4879S, Rab6 Antibody

Polyclonal Antibody for studying RAB6. Validated for Western Blotting. Highly specific and rigorously validated in-house, Rab6 Antibody (CST #4879) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Rab6 Antibody detects endogenous levels of total Rab6 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys133 of human Rab6 protein. Antibodies are purified by peptide affinity chromatography. Background Rab6 is a member of the Ras superfamily of small Rab GTPases implicated in endocytosis (1). The three distinct members of the Rab6 subfamily (Rab6A, Rab6A', and Rab6B) are structurally similar but likely exhibit non-overlapping functions (2,3). Rab6 localized to the Golgi (4) regulates retrograde transport of membrane-bound target proteins from the Golgi apparatus to the endoplasmic reticulum (5-7) or from the Golgi to the endosome during exocytotic transport (8). Rab6 interacts with microtubule motor proteins such as rabkinesin-6 (KIF20A) and dynein/dynactin complexes; Rab6-mediated transport requires a functionally intact microtubule system (9,10). Rab6 also regulates cytokinesis and cell cycle check point through interactions with Rab6 effector proteins, including the dynein/dynactin protein DCTN1 and the GTPase activating protein RABGAP1 (11,12). Alternate Names GTP-binding protein RAB6A'; GTP-binding protein RAB6B; Oncogene RAB6; Rab GTPase; Rab-6; RAB6; RAB6, member RAS oncogene family; RAB6A; Rab6A variant 3; RAB6A, member RAS oncogene family; RAB6A'; RAB6B; Ras-related protein Rab-6A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 24 SOURCE: Rabbit