CST, 48813SF, Neurofascin 155 (D7B6O) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying neurofascin. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Neurofascin 155 (D7B6O) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #48813) is ready to ship. Product Usage Information This product is the carrier free version of product #15035. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 15035 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Neurofascin 155 (D7B6O) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total neurofascin 155 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg881 of human neurofascin 155 protein. Background Myelinated axons contain un-myelinated gaps called nodes of Ranvier. These regularly spaced gaps are critical for the proper propagation and rapid conduction of nerve impulses in the central and peripheral nervous system (1). The structure and organization of the nodes of Ranvier is dictated by interaction between the axon and glial cells (2). Voltage-gated sodium channels concentrated at the nodes and potassium channels clustered at the paranodes are responsible for propagation of the action potentials (3,4). Other proteins that contribute to the architecture and function of the nodes of Ranvier include βIV spectrin (5), ankyrin-G (6), and the L1 cell adhesion molecules, neurofascin and NrCAM (7,8). Alternative splicing produces several neurofascin isoforms that differ in temporal and spatial expression. Neurofascin 186 is expressed in axons where it is concentrated at the nodes. Research studies indicate that neurofascin 186 is responsible for nodal assembly and clustering of sodium channels (9). Neurofascin 155 is expressed in glial cells and is localized to myelin paranodes. Interactions between neurofascin 155 and the contactin-associated protein (Caspr) tether the myelin sheath to the axon (10). N-linked glycosylation results in two forms of neurofascin 155 (high and low) that are differentially expressed during development (11). Alternate Names DKFZp686P2250; FLJ46866; KIAA0756; NEDCPMD; Neurofascin; neurofascin homolog; neurofascin homolog (chicken); NF; NFASC; NRCAML Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 140-155 Source/Isotype: Rabbit IgG