CST, 48836SF, EBF1 (E2I3Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying EBF1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, EBF1 (E2I3Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #48836) is ready to ship. Product Usage Information This product is the carrier free version of product #57794. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 57794 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity EBF1 (E2I3Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total EBF1 protein. This antibody may cross-react with EBF2 and EBF3. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly500 of human EBF1 protein. Background Early B-cell factor 1 (EBF1), also referred to as COE1, is a pioneering transcription factor that is crucial for defining B cell lineage. In hematopoiesis, EBF1 promotes the expression of genes that specify B cell lineage commitment, such as , , and , while also repressing the expression of transcription factor genes that promote alternate lineages (1,2). EBF1 has also been reported to play a role in olfactory neuronal cell, adipocyte, and early osteoclast differentiation (3-6). Due to the crucial role of EBF1 in B cell development, mutations in EBF1 can lead to the development of B-cell acute lymphoblastic leukemia (B-ALL) (7,8). EBF1 expression is also suppressed in some solid tumor cancers, including genomic loss in breast cancer and downregulation in pancreatic ductal adenocarcinomas (9,10). Lower expression levels of EBF1 also correlate with lower survival rates in patients with cholangiocarcinoma and colorectal carcinoma (CRC), and induced overexpression of EBF1 in CRC cell lines induces cell cycle arrest and apoptosis and (11,12). Alternate Names COE1; Collier, Olf and EBF transcription factor 1; early B cell factor 1; Early B-cell factor; early B-cell factor 1; EBF; EBF transcription factor 1; EBF1; FLJ39389; FLJ41763; O/E-1; OE-1; OLF1; olfactory neuronal transcription factor 1; Transcription factor COE1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG