CST, 48868S, IRF-3 (D9J5Q) Mouse Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying IRF-3. Validated for Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, IRF-3 (D9J5Q) Mouse Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #48868) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity IRF-3 (D9J5Q) Mouse Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human IRF-3 protein. This antibody recognizes residues surrounding Gly145 of human IRF-3 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). Alternate Names IIAE7; Interferon regulatory factor 3; IRF-3; IRF3 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1