CST, 48938T, alpha-Smooth Muscle Actin (1A4) Mouse Monoclonal Antibody (IF Formulated)

Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, alpha-Smooth Muscle Actin (1A4) Mouse Monoclonal Antibody (IF Formulated) (CST #48938) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Frozen): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity alpha-Smooth Muscle Actin (1A4) Mouse Monoclonal Antibody (IF Formulated) recognizes endogenous levels of total α-smooth muscle actin protein Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle actin protein. Background Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as "non-muscle" actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1). Alternate Names AAT6; ACTA; ACTA2; actin alpha 2, smooth muscle; actin, alpha 2, smooth muscle, aorta; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; ACTVS; alpha 2 actin; alpha smooth muscle Actin; Alpha-actin-2; alpha-cardiac actin; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; smooth muscle actin Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Mouse IgG2a