CST, 4893T, Nanog (1E6C4) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Nanog. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Nanog (1E6C4) Mouse Monoclonal Antibody (CST #4893) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:6400 - 1:25600 Immunofluorescence (Immunocytochemistry): 1:2000 Flow Cytometry (Fixed/Permeabilized): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Nanog (1E6C4) Mouse Monoclonal Antibody detects endogenous levels of total nanog protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human nanog protein. Background Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3). Alternate Names hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Mouse IgG1