CST, 48947S, BLNK (D3P2H) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying BLNK. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, BLNK (D3P2H) Rabbit Monoclonal Antibody (PE Conjugate) (CST #48947) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity BLNK (D3P2H) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total BLNK protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg282 of human BLNK protein. Background B cell linker protein (BLNK), also known as SLP-65 or BASH, is an adaptor molecule that plays key roles in B cell activation and B cell antigen receptor (BCR) engagement. BLNK acts at the interface between BCR-associated Syk and downstream signaling cascades (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its amino terminus and an SH2 domain at its carboxy terminus. After BCR ligation, BLNK is phosphorylated by Syk at multiple YXXP motifs, including Tyr72, Tyr84, Tyr96, and Tyr178 (1). These phosphorylated motifs provide docking sites for signaling molecules, such as BTK, PLCγ, and Vav. These signaling molecules bind to BLNK through their SH2 domains and together activate downstream signaling pathways (3,4). Through its SH2 domain, BLNK can also interact with tyrosine-phosphorylated targets, such as HPK1, thereby recruiting them to the BCR complex for signaling (5). Alternate Names AGM4; B cell adaptor containing SH2 domain; B cell linker; B cell linker protein; B-cell activation; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; B-cell linker; B-cell linker protein; BASH; bca; BLNK; BLNK-S; Cytoplasmic adapter protein; LY57; MGC111051; SLP-65; SLP65; Src homology [SH2] domain-containing leukocyte protein of 65 kD; Src homology 2 domain-containing leukocyte protein of 65 kDa Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG