CST, 4897S, Ajuba Antibody

Polyclonal Antibody for studying Ajuba. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Ajuba Antibody (CST #4897) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Ajuba Antibody detects endogenous levels of total Ajuba protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Ajuba. Antibodies are purified by protein A and peptide affinity chromatography. Background Ajuba belongs to the Zyxin/Ajuba family, members of which include LIMD1, LPP, Trip6 and Zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin/Ajuba family members associate with the actin cytoskeleton, are components of both cell-cell junction adhesive complex and integrin-mediated adhesive complex, and shuttle in and out of the nucleus (1). Ajuba is involved in Rac activation during cell migration and activates Aurora A in order to commit to mitosis (2-3). Ajuba and its family members also have an amino-terminal proline-rich pre-LIM region, and Ajuba augments ERK activation in a Ras-dependent manner (4). Alternate Names Ajuba; ajuba LIM protein; JUB; jub, ajuba homolog; jub, ajuba homolog (Xenopus laevis); LIM domain-containing protein ajuba; MGC15563; Protein ajuba Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit