CST, 48980T, NLRP2 (F3C9H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NLRP2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NLRP2 (F3C9H) Rabbit Monoclonal Antibody (CST #48980) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NLRP2 (F3C9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRP2 protein. This antibody cross-reacts with a 60 kDa protein of unknown identity in some cell lysates. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln679 of human NLRP2 protein. Background NOD-like receptor family pyrin domain-containing 2 (NLRP2) is a member of the NLR family, which is composed of proteins that can form inflammasomes and/or act as sensors that drive inflammation and cell death (1,2). They accomplish this by promoting maturation of downstream targets, such as caspase-1, IL-1b, IL-18, IL-37, and gasdermin D (3). NLRP2 has been shown to interact with apoptosis-associated speck-like protein containing a CARD (ASC), and its expression is induced in mouse dorsal root ganglia following treatment with complete Freund's adjuvant, which causes inflammation and pain (4,5). NLRP2 is also essential for embryonic development in mice, with depletion leading to early arrest (6). Alternate Names CLR19.9; FLJ20510; NACHT, leucine rich repeat and PYD containing 2; NACHT, LRR and PYD containing protein 2; NACHT, LRR and PYD domains-containing protein 2; NALP2; NBS1; NLR family pyrin domain containing 2; NLR family, pyrin domain containing 2; NLRP2; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 2; Nucleotide-binding site protein 1; PAN1; PYPAF2; PYRIN domain and NACHT domain-containing protein 1; PYRIN-containing APAF1-like protein 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG