CST, 4902S, PI4 Kinase Antibody

Polyclonal Antibody for studying PI4 Kinase alpha. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, PI4 Kinase Antibody (CST #4902) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PI4 Kinase Antibody detects endogenous levels of total PI4 Kinase isoform 230. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of PI4 Kinase. Antibodies are purified by protein A and peptide affinity chromatography. Background Inositol phospholipids have an important role in intracellular signaling in response to hormones, growth factors and neurotransmitters. Phosphatidylinositol 4-kinase phosphorylates phosphatidylinositol (PI) to phosphatidylinositol-4-phosphate (PIP). In a second step, PIP is further phosphorylated to phosphatidylinositol-4,5-bisphosphate (PIP2), and PIP2 is subsequently hydrolyzed by phospholipase C, producing the two intracellular second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). PI4K230, PI4K92 and PI4K55 are three PI4 kinase isoforms that have been characterized and classified according to their molecular weights of 230, 92 and 55 kD. Previously, PI4 kinases were classified into type II and III enzymes (2). All isoforms are located on distinct membranes and cellular compartments suggesting various tasks. PI4K230 is located at the endoplasmatic reticulum and outer membranes of mitochondria, PI4K92 at the Golgi apparatus and endoplasmatic reticulum, and PI4K55 at the plasma membrane and endosomes (3-5). PI4K230 is predominantly expressed in brain and moderately sensitive to wortmannin as well as specifically and irreversibly inhibited by cyclitol derivatives (3, 6). Alternate Names FLJ16556; phosphatidylinositol 4-kinase 230; Phosphatidylinositol 4-kinase alpha; phosphatidylinositol 4-kinase III alpha; phosphatidylinositol 4-kinase IIII+/-; phosphatidylinositol 4-kinase, catalytic, alpha; phosphatidylinositol 4-kinase, type III, alpha; PI4-kinase alpha; PI4K-alpha; pi4K230; PI4KA; PIK4; PIK4CA; PMGYCHA; PtdIns-4-kinase alpha; testicular secretory protein Li 35 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 230 SOURCE: Rabbit