CST, 4910S, Phospho-Wee1 (Ser642) (D47G5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Wee1 (Ser642) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Wee1 (Ser642) (D47G5) Rabbit Monoclonal Antibody (CST #4910) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Wee1 (Ser642) (D47G5) Rabbit Monoclonal Antibody detects endogenous levels of wee1 protein only when phosphorylated at Ser642. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser642 of human wee1. Background Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-7). Akt/PKB-dependent phosphorylation at Ser642 promotes a change in Wee1 localization from nuclear to cytoplasmic and is associated with G2/M arrest (8). Alternate Names DKFZp686I18166; FLJ16446; WEE1; WEE1 G2 checkpoint kinase; WEE1 homolog; Wee1-like protein kinase; WEE1+ homolog; WEE1A; Wee1A kinase; WEE1hu Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG