CST, 49114S, UHRF2 (E5G5P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UHRF2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, UHRF2 (E5G5P) Rabbit Monoclonal Antibody (CST #49114) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UHRF2 (E5G5P) Rabbit Monoclonal Antibody recognizes endogenous levels of total UHRF2 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala154 of human UHRF2 protein. Background Ubiquitin-like PHD and RING finger domain-containing protein 2 (UHRF2), also known as Np95/ICBP90-like RING finger protein (NIRF), is a multi-domain member of the UHRF family and a UHRF1 paralog. Like UHRF1, UHRF2 carries out a variety of biological functions through its multiple domains, including a ubiquitin-like (UBL) domain, tandem Tudor domain (TTD), plant homeodomain (PHD), SET and RING finger-associated (SRA) domain, and really interesting new gene (RING) finger domain (1). Both UHRF1 and UHRF2 localize primarily with highly methylated pericentromeric heterochromatin and specifically recognize H3K9me2/3 marks (2,3). However, although they share high structural and sequence homology, UHRF1 and UHRF2 do not always carry out redundant biological functions. For instance, both UHRF1 and UHRF2 associate with hemi-methylated DNA through their respective SRA domains and complex with DNMT1, but UHRF2 does not target DNMT1 to replication forks and therefore cannot compensate for methylation defects in UHRF1- mouse ES cells (3-5). UHRF1 and UHRF2 also exhibit opposing expression patterns in differentiating cells and various tissues, further suggesting separate functions (6,7). UHRF2 may function as a novel cell cycle regulator by interacting with key proteins such as cyclin A2, CDK2, p53, and PCNA (7,8). Aberrant UHRF2 expression has also been implicated in several cancers, including lung and colorectal cancer as well as hepatocellular carcinoma (9-11). Alternate Names DKFZp434B0920; DKFZp686G0837; E3 ubiquitin-protein ligase UHRF2; MGC33463; NIRF; Np95-like RING finger protein; Np95/ICBP90-like RING finger protein; Nuclear protein 97; Nuclear zinc finger protein Np97; RING finger protein 107; RING-type E3 ubiquitin transferase UHRF2; RNF107; TDRD23; ubiquitin like with PHD and ring finger domains 2; Ubiquitin-like PHD and RING finger domain-containing protein 2; ubiquitin-like with PHD and ring finger domains 2; ubiquitin-like with PHD and ring finger domains 2, E3 ubiquitin protein ligase; Ubiquitin-like-containing PHD and RING finger domains protein 2; ubiquitin-like, containing PHD and RING finger domains, 2; UHRF2; URF2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG