CST, 49120S, PTMScan® Control Peptides Multi-Pathway

Cell Extracts for studying in the research area. Product Usage Information Use with Cell Signaling Technology's PTMScan ® kit protocol from the Immunoaffinity Purification (IAP) step. Because the optimal amount of PTMScan ® Control Peptides Multi-Pathway for each user's experiments will depend on unique factors, such as mass spectrometer sensitivity, users may dilute these control peptides as needed. 1. Aliquot PTMScan ® Control Peptides Multi-Pathway for storage as single-use units at -20°C or proceed to immediate usage.  2. Resuspend sample peptides in the appropriate buffer and volume, e.g., 1.4 mL of PTMScan ® IAP Buffer (1X). 3. Clear sample peptides by centrifugation. 4. Transfer clarified sample peptides to tubes containing IAP beads. 5. Add 10 µL of PTMScan ® Control Peptides Multi-Pathway to IAP beads and sample peptides and mix well. 6. Continue with PTMScan ® workflows at the 2-hour incubation step. 7. Detect PTMScan ® Control Peptides Multi-Pathway in the LCMS data file. Storage This product is stable for 24 months when stored at -20°C. Aliquot to avoid multiple freeze/thaw cycles. Background The PTMScan Multi-Pathway Enrichment Kit #75676 introduces Cell Signaling Technology's PTMScan Direct Multi-Pathway proteomics service to the end user in a convenient and easy-to-use kit format. Based on the patented PTMScan method, the kit includes an array of site-specific antibodies conjugated to protein A beads for peptide immunoaffinity purification. The kit is designed for the screening, discovery, and quantitation of thousands of proteins and phosphorylation sites on hundreds of critical signaling proteins spanning multiple signaling pathways. These include cell cycle and checkpoint control, PI3K/Akt signaling, MAPK and JNK cascades, T cell and B cell receptor signaling, as well as some housekeeping targets. The kit can also be used as the enrichment reagent for customized targeted assays by including heavy isotope-labeled synthetic peptides to a subset of validated targets.