CST, 4913S, Ubc12 Antibody

Polyclonal Antibody for studying UBC12. Validated for Western Blotting. Highly specific and rigorously validated in-house, Ubc12 Antibody (CST #4913) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ubc12 Antibody detects endogenous levels of total Ubc12 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding K36 of human Ubc12. Antibodies are purified by peptide affinity chromatography. Background Similar to ubiquitin, NEDD8 is covalently linked to target proteins through an enzymatic cascade composed of NEDD8-specific E1 (activating)- and E2 (conjugating)-enzymes (1,2). The E2 ligase specific for NEDD8 is Ubc12 (3-5). Ubc12 forms a heterodimeric conjugate with NEDD8 in order to catalyze the transfer of NEDD8 from E1 to lysine side chains of target proteins (1,2). Well known targets of NEDD8 are cullin-based RING E3 ligases. Neddylation of cullin isoforms activates the related ubiquitin E3 complex by promoting its interaction with a cognate ubiquitin-E2 ligase (6-7). Neddylation of Cul-1 complexes containing βTrCP and SKP2 has been shown to be required for controlling the stability of important signaling targets such as IκB, NF-κB, and p27 Kip (8-10), thereby regulating cell cycle progression, signaling cascades, and developmental programming processes (11). Alternate Names epididymis secretory sperm binding protein; hUbc12; NEDD8 carrier protein; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; UBC-RS2; UBC12; UBE2M; ubiquitin carrier protein M; ubiquitin conjugating enzyme E2 M; ubiquitin conjugating enzyme E2M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 21 SOURCE: Rabbit