CST, 4916S, STIM1 Antibody

Polyclonal Antibody for studying STIM1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, STIM1 Antibody (CST #4916) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity STIM1 Antibody detects endogenous levels of total STIM1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human STIM1. Antibodies are purified by protein A and peptide affinity chromatography. Background Ca is a key second messenger in many intracellular signaling pathways. Ca signals control many cellular functions ranging from short-term responses such as contraction and secretion to longer-term regulation of cell growth and proliferation (1,2). Stromal interaction molecules (STIMs) function as Ca sensors that detect changes in Ca content in intracellular Ca stores (3). STIM1 is conserved, ubiquitously expressed, and functions as an endoplasmic reticulum (ER) Ca sensor that migrates from the ER Ca store to the plasma membrane where it activates calcium-release-activated calcium (CRAC) channels when the ER Ca store is low (4). STIM1 is a potential tumor suppressor; defects in STIM1 may cause rhabdomyosarcoma and rhabdoid tumors (5). STIM1 can either homodimerize or form heterodimers with STIM2. STIM2 possesses a high sequence identity to STIM1 and can function as an inhibitor of STIM1-mediated plasma membrane store-operated Ca entry (6). However, further investigation is required to elucidate the true physiological function of STIM2. Alternate Names D11S4896E; GOK; IMD10; STIM1; STRMK; Stromal interaction molecule 1; TAM; TAM1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 85 SOURCE: Rabbit