CST, 49226S, FGL1 Antibody

Polyclonal Antibody for studying FGL1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, FGL1 Antibody (CST #49226) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FGL1 Antibody recognizes endogenous levels of total FGL1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn69 of human FGL1 protein. Antibodies are purified by peptide affinity chromatography. Background FGL1 (fibrinogen-like protein 1, also known as hepassocin) is a secreted protein mainly expressed in liver and its expression is further enhanced following acute liver injury (1-3). FGL1 null mice have multiple metabolic abnormalities and are more prone to develop hepatocellular carcinoma under certain experimental conditions, suggesting FGL1 has diverse functions (4,5). More recently, FGL1 was found to be highly expressed in human cancer cells and function as an inhibitory ligand for LAG3. Blockade of the FGL1-LAG3 interaction stimulates tumor immunity in mouse models. In addition, a high plasma FGL1 level in human cancer patients is associated with a poor prognosis and resistance to anti-PD-1/PD-L1 immunotherapy (6). Alternate Names FGL1; fibrinogen like 1; fibrinogen-like 1; Fibrinogen-like protein 1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1; HP-041; HPS; LFIRE-1; LFIRE1; Liver fibrinogen-related protein 1; MGC12455 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 32 SOURCE: Rabbit