CST, 4923T, Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying 4E-BP2 (Thr46) non-phosphate/4E-BP1 (Thr46) non-phosphate/4E-BP3 (Thr32) non-phosphate. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit Monoclonal Antibody (CST #4923) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit Monoclonal Antibody detects endogenous levels of 4E-BP1 only when dephosphorylated at Thr46. This antibody cross-reacts with 4E-BP2 and 4E-BP3 dephosphorylated at equivalent sites. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr46 of human 4E-BP1. Â Background Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). Alternate Names 4E-BP1; 4E-BP2; 4E-BP3; 4EBP1; 4EBP2; 4EBP3; BP-1; eIF4E-binding protein 1; eIF4E-binding protein 2; eIF4E-binding protein 3; EIF4EBP1; EIF4EBP2; EIF4EBP3; eukaryotic initiation factor 4E-binding protein 3; eukaryotic translation initiation factor 4E binding protein 1; eukaryotic translation initiation factor 4E binding protein 2; eukaryotic translation initiation factor 4E binding protein 3; Eukaryotic translation initiation factor 4E-binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 2; Eukaryotic translation initiation factor 4E-binding protein 3; MGC4316; PHAS-I; PHASII; Phosphorylated heat- and acid-stable protein regulated by insulin 1; phosphorylated, heat and acid stable regulated by insulin protein II Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 15-20 Source/Isotype: Rabbit IgG