CST, 49243SF, CD206/MRC1 (E2L9N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MRC1. Validated for WB,IHC,IHC,IF. Highly specific and rigorously validated in-house, CD206/MRC1 (E2L9N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #49243) is ready to ship. Product Usage Information This product is the carrier free version of product #91992. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 91992 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD206/MRC1 (E2L9N) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD206/MRC1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human CD206/MRC1 protein. Background The mannose receptor (MR/CLEC13D/CD206/MMR/MRC1/Macrophage mannose receptor 1) is an endocytic receptor expressed by populations of dendritic cells, macrophages, and nonvascular endothelium (1). The mannose receptor is a heavily glycosylated type I transmembrane protein with three types of extracellular domains and a short carboxy-terminal cytoplasmic domain with no apparent signaling motif (2-4). The extracellular portion of the protein is made up of a CR domain, which binds sulfated glycans, an FNII domain, which binds collagens, and eight C-type lectin domains, which bind carbohydrates containing mannose, fucose, or GlcNAc (4-7). The receptor recycles between the plasma membrane and early endosomes (8). Functions include a role in antigen cross-presentation, clearance of endogenous proteins, pathogen detection, and trafficking through lymphatic vessels (9-12). The mannose receptor is a marker for M2 macrophages (13). Alternate Names bA541I19.1; C-type lectin domain family 13 member D; C-type lectin domain family 13 member D-like; CD206; CLEC13D; CLEC13DL; hMR; Human mannose receptor; Macrophage mannose receptor 1; Macrophage mannose receptor 1-like protein 1; Mannose receptor; mannose receptor C type 1; mannose receptor C-type 1; mannose receptor, C type 1; mannose receptor, C type 1-like 1; MMR; MRC1; MRC1L1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 190-250 Source/Isotype: Rabbit IgG