CST, 49253T, DIMT1 (E7O5R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Dimt1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DIMT1 (E7O5R) Rabbit Monoclonal Antibody (CST #49253) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DIMT1 (E7O5R) Rabbit Monoclonal Antibody recognizes endogenous levels of total DIMT1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly304 of human DIMT1 protein. Background Dimethyladenosine transferase 1 (DIMT1), also known as DIMT1L, is a methyltransferase responsible for the dimethylation of adenosines on the 18S rRNA subunit. Binding of DIMT1 to the pre-rRNA is required for proper processing of the 18S subunit (1). Independent of its catalytic activity, DIMT1 seems to function as a scaffold protein that is essential for the assembly of the 40S rRNA subunit (2). DIMT1 also has been shown to have higher expression in certain cancer types, including multiple myeloma and gastric cancer (3,4). Alternate Names 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase; 18S rRNA dimethylase; DIM1; DIM1 dimethyladenosine transferase 1 homolog; DIM1 dimethyladenosine transferase 1-like; DIM1 dimethyladenosine transferase 1-like (S. cerevisiae); dimethyladenosine transferase; DIMT1; DIMT1 rRNA methyltransferase and ribosome maturation factor; DIMT1L; HSA9761; HUSSY5; Probable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase; Probable 18S rRNA dimethylase; Probable dimethyladenosine transferase; Probable S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG