CST, 49256S, SAMD9 (F9J1K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SAMD9. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SAMD9 (F9J1K) Rabbit Monoclonal Antibody (CST #49256) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SAMD9 (F9J1K) Rabbit Monoclonal Antibody recognizes endogenous levels of total SAMD9 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala95 of human SAMD9 protein. Background Innate immunity provides the first line of defense against infectious microbial pathogens through a family of proteins known as pattern-recognition receptors (PRRs), which detect pathogen-associated molecular patterns (PAMPs) consisting of nucleic acids, peptides, peptidoglycans, and lipopolysaccharides (1-4). Sterile alpha motif domain-containing 9 (SAMD9) plays a role in innate immunity by recognizing both cytosolic double-stranded DNA and RNA and functions broadly as an antiviral protein (2-4). SAMD9 has been most studied in the innate immune response to infection by poxviruses (3,4). SAMD9 is evolutionarily conserved, and mutations in human cause a severe multi-system disorder called the MIRAGE syndrome (5-7). SAMD9 contains an N-terminal SAM domain, a nucleic acid binding domain, and a putative STAND (signal transduction adenosine triphosphatases with numerous associated domains) domain (1,8). STAND proteins share a set of conserved domains: a C-terminal PAMPs sensor, a structural NOD domain, and an N-terminal effector that has been shown to bind double-stranded nucleic acids (8). In addition to its antiviral activity, SAMD9 has also been studied in cancer and autoimmune disease. SAMD9 has been shown to act as a tumor suppressor in lung, colon, and breast cancers (9), and has increased expression in rheumatoid arthritis (10). The roles of SAMD9 in innate immunity, autoimmunity, and cancer have resulted in increased research interest as a therapeutic target. Alternate Names C7orf5; DRIF1; expressed in aggressive fibromatosis; FLJ20073; KIAA2004; MIRAGE; NFTC; OEF1; OEF2; SAM domain-containing protein 9; SAMD9; sterile alpha motif domain containing 9; Sterile alpha motif domain-containing protein 9 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 190 Source/Isotype: Rabbit IgG