CST, 4928S, Granzyme A Antibody

Polyclonal Antibody for studying Granzyme A. Validated for Western Blotting,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, Granzyme A Antibody (CST #4928) is ready to ship. Product Usage Information Western Blotting: 1:1000 Peptide ELISA (DELFIA): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Granzyme A Antibody detects endogenous levels of Granzyme A protein. No cross-reactivity was observed with Granzyme B. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding threonine 121 of human Granzyme A. Antibodies were purified by protein A and peptide affinity chromatography. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Alternate Names CTL tryptase; CTLA3; Cytotoxic T-lymphocyte proteinase 1; Cytotoxic T-lymphocyte-associated serine esterase-3; Fragmentin-1; GRAA; granzyme 1; Granzyme A; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme-1; GZMA; H factor; Hanukah factor serine protease); Hanukkah factor; HF; HFSP Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit