CST, 49307S, Gasdermin A Antibody

Polyclonal Antibody for studying Gasdermin A. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Gasdermin A Antibody (CST #49307) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Gasdermin A Antibody recognizes endogenous levels of total Gasdermin A protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly256 of human Gasdermin A protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The gasdermin family, which includes GSDMA, GSDMB, GSDMC, GSDMD, and GSDME, has been shown to play a role in inflammation and cell death. Gasdermin D has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7). Gasdermin A (GSDMA) is preferentially expressed in the epithelium of the skin and gastrointestinal tract and is frequently suppressed in gastric cancer (8-10). Mice express three Gasdermin A genes termed (8). The role of Gasdermin A has been associated with cellular differentiation, apoptosis, pyroptosis, and autophagy (10-15). Expression of an N-terminal fragment of , but not the full-length protein, induces pyroptosis, but the mechanisms of any cleavage of are unknown (12). The most widely studied mouse form, , is expressed in mouse keratinocytes and is associated with skin differentiation and inflammation, and a dominant mutation has been found to play a causative role in alopecia (16,17). Alternate Names FKSG9; FLJ39120; gasdermin 1; gasdermin A; Gasdermin-1; Gasdermin-A; GSDM; GSDM1; GSDMA; MGC129596 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 49 SOURCE: Rabbit