CST, 49332S, Phospho-MST1 (Thr183)/MST2 (Thr180) (E7U1D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MST1 (Thr183) phosphate/MST2 (Thr180) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-MST1 (Thr183)/MST2 (Thr180) (E7U1D) Rabbit Monoclonal Antibody (CST #49332) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-MST1 (Thr183)/MST2 (Thr180) (E7U1D) Rabbit Monoclonal Antibody recognizes endogenous levels of MST1 and MST2 protein only when phosphorylated at Thr183 and Thr180, respectively. Due to sequence similarities, this antibody may also recognize MST3 (STK24) and MST4 (STK26), but only when phosphorylated at Thr190 and Thr178, respectively. Based on their predicated molecular weights, MST3 and MST4 are not expected to co-migrate with MST1 and MST2. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr183 of human MST1 protein. Background Mammalian sterile-20-like (MST) kinases are upstream regulators of mitogen-activated protein kinase (MAPK) signaling pathways that regulate multiple cellular processes, including proliferation, apoptosis, migration, and cytoskeletal rearrangement (1). This family of serine/threonine kinases includes MST1 (STK4) and MST2 (STK3), two functionally related proteins with conserved amino-terminal kinase domains and carboxy-terminal regulatory domains that contain nuclear export signals (1-3). During apoptosis, caspase-mediated cleavage of MST1/2 removes the inhibitory regulatory domain, triggering autophosphorylation and activation of the kinase domain, which is translocated to the nucleus. Nuclear translocation of the active kinase induces chromatin condensation and other events associated with apoptotic progression (4). Research studies indicate that MST1/2 are orthologous to Hippo (Hpo), one of the core regulatory proteins in the Hippo signaling pathway. This evolutionarily conserved program controls tissue growth and organ size by regulating cell proliferation, apoptosis, and stem cell self-renewal. The mammalian Hippo signaling pathway involves a kinase cascade, where the MST1/2 kinases and the SAV1 scaffold protein form a complex that leads to phosphorylation and activation of LATS1/2. The LATS1/2 kinases phosphorylate YAP and TAZ, promoting cytoplasmic sequestration and inhibition of these transcription coactivators (5). Alternate Names dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2)); DKFZp686A2068; epididymis secretory sperm binding protein; FLJ90748; hippo homolog; KB-1458E12.1; kinase responsive to stress 2; KRS1; KRS2; Mammalian STE20-like protein kinase 1; Mammalian STE20-like protein kinase 2; mammalian sterile 20-like 1; MST-1; MST-2; MST1; MST1/C; MST1/N; MST2; MST2/C; MST2/N; serine/threonine kinase 3; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); serine/threonine kinase 4; Serine/threonine-protein kinase 3; Serine/threonine-protein kinase 3 20kDa subunit; Serine/threonine-protein kinase 3 36kDa subunit; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase 4 18kDa subunit; Serine/threonine-protein kinase 4 37kDa subunit; Serine/threonine-protein kinase Krs-1; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; STE20-like kinase MST2; STK3; STK4; yeast Ste20-like; YSK3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 59 Source/Isotype: Rabbit IgG