CST, 49334SF, NDRG4 (D4A6) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NDRG4. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, NDRG4 (D4A6) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #49334) is ready to ship. Product Usage Information This product is the carrier free version of product #9039. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 9039 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NDRG4 (D4A6) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total NDRG4 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly217 of human NDRG4 protein. Background The NDRG (N-Myc downstream-regulated gene) family consisting of NDRG1, NDRG2, NDRG3, and NDRG4 are structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was upregulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues and have been suggested to function as a tumor suppressors (4,5). Unlike other members of the family that are widely expressed, NDRG4/Bdm1 expression is restricted primarily to the heart and brain (2,3,6,7). Multiple alternatively spliced isoforms of NDRG4 have been reported (3,7). Expression of NDRG4 is reduced in the brain of patients with Alzheimer's disease (3). In contrast, NDRG4 expression is elevated in glioblastoma and contributes to cell cycle progression and survival (8). NDRG4 also may be inactivated by promotor methylation in colorectal cancer and function as a tumor suppressor gene (9). Alternate Names BDM1; Brain development-related molecule 1; DKFZp686I1615; FLJ30586; FLJ42011; KIAA1180; MGC19632; N-myc downstream-regulated gene 4 protein; NDRG family member 4; NDRG4; Protein NDRG4; SMAP-8; SMAP8; smooth muscle-associated protein 8; Vascular smooth muscle cell-associated protein 8 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 37-45 Source/Isotype: Rabbit IgG