CST, 4933S, Claudin-1 Antibody

Polyclonal Antibody for studying Claudin-1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Claudin-1 Antibody (CST #4933) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:25 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Claudin-1 Antibody detects endogenous levels of total claudin-1 protein. Based on sequence similarity, this antibody may cross-react with claudin-2. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of mouse claudin-1. Antibodies are purified using protein A and peptide affinity chromatography. Background Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6). Alternate Names claudin 1; Claudin-1; claudin1; CLD1; CLDN1; ILVASC; SEMP1; Senescence-associated epithelial membrane protein; senescence-associated epithelial membrane protein 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20 SOURCE: Rabbit